What happens if I connect my card to a PCI Express or x16 slot

Explaining PCIe Slots pcie 2 0 x16 slot Pci express version vs version , Gen 2 vs Gen 3 Is there any real performance loss when pcie 4 gpu in pcie 3 slot Hi everyone, I want to upgrade my custom build TrueNAS to 10GbE However, I find that the motherboard only remain a PCIe x1 (in x16

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