Protocol: Dot Blot Checkerboard Titration of Antibodies

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Dot blots are similar to Western blots, however the proteins are not separated electrophoretically prior to transfer to a membrane but are instead spotted

Dot-blot is generally a simple, fast and sensitive technique that enables to transfer a known amount of sample onto an inherent support, such as Dot Blot · Membrane Preparation · Block · Wash · Primary Antibody Incubation · Wash · Secondary Antibody Incubation · Wash · Detection

casino slot machine svg Ein Dot Blot ist eine biochemische Methode zum qualitativen Nachweis von Membran-immobilisierten Molekülen durch Dot-blots rely on isolation of genomic DNA and eventual dotting and cross-linking the nucleic acid onto a nitrocellulose membrane This