Does it Matter Which PCI-E SLOT you Use?

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The different slot types available are an important consideration when buying a graphics card or computer This guide describes the differences between these

Echo II DV Desktop NEW Desktop 2-slot, dual-Thunderbolt interface to PCIe card expansion system For computers with Thunderbolt 4 or Thunderbolt 3 ports  Dual For SSD Two of the world's fastest slots One delivers up to PCIe Gen3 x4 32Gbs transfer speed, the other one supports SATA3

lucky punch slot 2 slots for today's teeny-tiny SSDs are between the PCIe slots…which means that the drives sit right under my graphics card In terms of thermal Slot 1357 are x16 slots, the other slots are x8 only Could I use the x8 slots too without to lost to much GPU power? I would like to